Givotia Moluccana Analysis

Givotia Moluccana Analysis MATERIALS AND METHODS 4.1. PLANT MATERIAL 4.1.1. COLLECTION OF PLANT The plant aerial parts of Givotia moluccanawas collected and Authentified by Dr. K. Madhava Chetty, Department of Botany, Sri Venkateswara University, Tirupathi (AP). 4.1.2. PREPARATION OF THE EXTRACT The dried leaves of G. moluccana was collected, cleaned, dried and powdered in a grinder  ­Ã‚ ­- mixer to obtain a coarse powder and then passed through 40 mesh sieve. About 1000 gm of powdered drug was extracted with aqueous ethanol by soxhlet apparatus. The extraction was carried out until the drug becomes exhausted. The solvent was recovered from their extract by distillation under reduced pressure. The dried extract thus obtained was kept in a desicator and was used for further experiments. 4.2. EXPERIMENTAL ANIMALS Healthy adult male wistar rats weighing between 150-200gm were used for the present study. The animals were housed in groups of six and maintained under standard conditions (27 ±2 ºC, relative humidity 44 56% and light and dark cycles of 10 and 14 hours respectively) and fed with standard rat diet and purified drinking water ad libitum for 1 week before and during the experiments. All experiments and protocols described in present study were approved by the Institutional Animal Ethical Committee (IAEC) of P.Rami Reddy Memorial Collage of Pharmacy (1423/PO/a/11/CPCSEA/102/2014). All the experiments were performed in the morning according to current guidelines for the care of laboratory animals and the ethical guidelines for the investigation of experimental pain in conscious animals (Zimmerman, 1983). 4.3. DRUGS AND CHEMICALS Epinephrine, DTNB, Triphenyl tetrazolium chloride and isoproterenol were obtained from Sigma-Aldrich, Bangalore. Thiobarbituric acid (TBA), trichloro acetic acid, hydrogen peroxide were obtained from SD fine chemicals Ltd Mumbai. Sodium dihydrogen phosphate, potassium dihydrogen phosphate, tris buffer and all other reagents used were of analytical grade. CK-MB, LDH, SGOT, SGPT, ALP, Total cholesterol, HDL, and triglyceride estimation kits were obtained from Erba diagnostic Ltd. India. 4.4. INSTRUMENTS Analytical Auto analyzer (MaxLyzer NB-201), UV-Visible spectrophotometer (Shimadzu, Model no: 2203), Electronic balance (Shimadzu, Model no: DS-852 J), Tissue homogeniger (Ever shine, Model no: 607), Remi centrifuge (Remi, Model no: KKLO-9013). 4.5 ACUTE ORAL TOXICITY STUDY The acute oral toxicity study was done according to OECD 423 guidelines. Wistar albino rats of either sex were selected randomly and divided into six groups (n = 6). The animals were fasted overnight and extract in doses of 100, 250, 500, 1000, 2000 and 5000 mg/kg body weight, were administered orally to II – VI groups. Group I which received vehicle (CMC) served as control. The animals were observed continuously for 2 hr, and then intermittently for 6 hr and at the end of 24 hours, the number of deaths was noted to determine LD50 of the extract (Annie et al., 2004). 4.6. EXPERIMENTAL DESIGN 4.6.1. NEPHROPROTECTIVE ACTIVITY The experimental animals were randomly divided in to 5 groups (n= 6) and treated for duration of 21 days as per the treatment schedule given in table no: 3. Nephrotoxicity was induced by administration of Gentamycin (80 mg/kg I.P) daily for 7 days. Ethanolic extract of G. moluccanawas freshly suspended in CMC and administered to animals by oral feeding needle. Table no: 3 Treatment schedule –Evaluation of nephroprotective activity of EEGM against gentamycin induced nephrotoxicity in Wistar Rats. I.P = Intra peritoneal, P.O = Per oral. 4.6.2. COLLECTION OF BLOOD AND URINE SAMPLES The blood samples were collected from the retrorbital venous plexus of rats without any coagulant for the separation of serum, at the regular intervals of the treatment. After collecting the blood in effindraf tubes they were kept for 1 h at room temperature and serum was separated by centrifugation at 2000 rpm for 15 min and stored until analyzed for various biochemical parameters. Urine was collected over 24 hours on the 21st day by keeping the test animals in metabolic cages. The volume of collected urine samples was measured followed by estimation of biochemical parameters, namely urine Creatinine, urine uric acid and urine urea. 4.7. PARAMETERS MONITERED 4.7.1. BIOCHEMICAL ESTMATIONS i. Estimation of Urea (Berthelot Method) Principle: The reaction sequence employed in the assay is as follows: Urea + H2O Urease 2NH3 + CO2 NH3 + Salicylate +Hypochlorite Nitropruside 2-2-Dicarboxy Indophenol Urease catalyses the conversion of Urea to Ammonia and Carbondioxide. The ammonia released reacts with a mixture of Slicylate. Hypochlorite and Nitropruside to yield a blue-green colored compound (Indophenol). The intensity of color produced is proportional to the concentration of urea in the sample and is measured photometrically at 578 nm or with yellow filter. Reagent preparation: Transfer the entire Enzyme Concentrate (1A) into Urease Reagent (1) with the dropper (or) microtip provided. Assay Procedure: Pipette into test tubes labeled Blank (B), Standard(S), Test(T) as follows. Mix and Read absorbance of Standard (S) and Test (T) against Blank (B) at 578 nm (570-620 nm) or with yellow filter. The final color is stable for 30 min. at R.T. Calculations: Blood urea nitrogen in mg/dl = a X 0.467 Urine Urea in gm/24 hours = a X 24 hrs urine volume in litres. ii. Estimation of BUN (GLDH-Urease Method) Methodology : Talke and Schubert, Tiffany et al. Principle: The estimation of Urea in serum involves the following enzyme catalyzed reactions: Urea + H2O Urease 2NH3 + CO2 NH3 + ÃŽ ±-KG + NADH GLDH Glutamate + NAD ÃŽ ±-KG : ÃŽ ±-Ketoglutarate GLDH : Glutamate dehydrogenase The rate of decrease in absorbance is monitored at 340 nm and is directly proportional to urea concentration in the sample. Procedure: Mix well, and aspirate standard followed by samples. Calculation: Determine absorbance change (ΔA) for the standard and unknown samples by using the formula. ΔA = A1 – A2 Urea = ΔA of Test Concentration of (mg/dl) ΔA OF Standard Standard (mg/dl) iii. Estimation of Uric acid (Uricase/POD) Principle: Uric acid is oxidized to Allontoin and hydrogenperoxide by the enzyme uricase. In presence of peroxidase, released hydrogen peroxide is coupled with Aniline derivative and 4-amino antipyrine (4-aap) to form colored chromogen complex. Absorbence of colored dye is measured at 550 nm and is proportional to Uric acid concentration in the sample (Schultz, 1984; Teivedi et al., 1978). Uric acid + 2H2O Uricase Allontoin + CO2 + H2O2 H2O2 + Aniline derivative + 4-AAP POD Chromogen complex + H2O2 Procedure: Mix well. Incubate at 37 ºC for 5 minutes. Programme the analyzer as per assay parameters. Blank the analyzer with reagent blank. Measure absorbance of standard followed by the test. Calculate results as per given calculation formula. Calculations: Serum/plasma/uric acid = Absorbance of Test 6 (mg/dl) Absorbance of Standard Urine uric acid = Dilution 24 hours urine volume in dl. Factor (mg/day) Conversion factor: Uric acid concentration in mmol/L = Uric acid in mg/dL 0.059 iv. Estimation of Creatinine (Mod. Jaffes Kinetic Method) Principle: Picric acid in an alkaline medium reacts with creatinine to form an orange coloured complex with the alkaline picrate. Intensity of the colour formed during the fixed time is directly proportional to the amount of creatinine present in the sample. Creatinine + Alkaline Picrate Orange Coloured Complex Procedure: Pipette into clean dry test tubes labeled as Standard (S) or Test (T): Mix well and read the initial absorbance A for the Standard and Test 1 after exactly 30 seconds. Read another absorbance A of the Standard 2 and Test exactly 60 seconds later. Calculate the change in absorbance ΔA for both the Standard and Test. For Standard Δ AS = A2 S – A1 S For Test Δ AT = A2 T – A1 T Calculations: Creatinine in mg/dl = 2.0 Urine Creatinine in g/L = x 1.0 Urine Creatinine g/24 Hrs. = Urine Creatinine in g/L x Vol. of urine in 24 Hrs. v. Estimation of Total Protein (Biuret Method ) Methodology: The peptide bonds of protein react copper ions in alkaline solution to form blue-violet complex, (biuret reaction). Each copper ion complexing with 5 or 6 peptide bonds. Tartarate is added as a stabilizer whilst iodide is used to prevent auto-reduction of the alkaline copper complex. The color formed is proportional to the protein concentration and is measured at 546nm (520-560nm). Procedure: Incubate for 10 minutes at 37 º C. Read absorbance of the standard and each test at 546 nm( 520-560 nm) against reagent blank. Calculations: Calculate the results as follows: Total Protein = Absorbance of Test Concentration of (g/dl) Absorbance of Standard Standard (g/dl) vi. Estimation of Albumin (Bromocresol Green) Principle: At pH 3.68, Albumin acts as a cation and binds to the anionic dye Bromocresol Green (BCG),forming a green colored complex. The color intensity of the complex is proportional to Albumin concentration in the sample (Gendler Proteins, 1984; Gustsfsson, 1978). Albumin + BCG Ph 3.68 Green colored complex. Procedure: Mix well. Incubate at Room Temperature (15-30 ºC) for 1 minute. Programme the analyzer as per assay parameters. Blank the analyzer with reagent blank. Measure absorbance of standard followed by the test. Calculate results as per given calculation formula. Calculations: Albumin (g/dL) = Absorbance of Test 4 Absorbance of Standard Globulin = Total Protein Albumin Conversion factor: Albumin concentration in g/L = Albumin concentration in g/dL 10 vii. Estimation of Cholestrol (CHOD-PAP Method) Methodology: Modified Roeschlau,s Method Principle: The estimation of cholesterol involves the following enzyme catalyzed reactions. Cholestrol ester CE Ckolestrol + Fatty acid Cholestrol + O2 CHOD Cholest-4-en-3-one + H2O2 2H2O2 + 4AAP + Phenol POD 4H2O + Quinoneimine CE : Cholestrol esterase CHOD : Cholestrol Oxidase 4AAP : 4-Aminoantipyrine Procedure: Mix well and incubate at 370C for 10 minutes. Aspirate Blank followed by Standard and Tests. Read the absorbance of standard and each test tube against blank at 505 nm or 505/670 nm on bichromic analyzer. Calculations: Cholestrol (mg/dL) = Absorbance of Test Concentration of Standard (mg/dl) Absorbance of Standard viii. Estimation of Glucose (GOD POP Method) Methodology: Trinder, s Method. Principle: Gucose + O2 + H2O Glucose oxidase Gluconic acid + H2O2 H2O2 + 4HBA + 4AAP Peroxidase Quinonemine Dye + 2 H2O 4AAP : 4-Aminoantipyrine 4HBA : 4-Hydroxy benzoic acid. The intensity of the pink color formed is proportional to the glucose concentration and can be measured photometrically between 500 to 540 nm. Procedure: Mix well and incubate for 10 minutes at 370 C. Read the absorbance of standard and each test tube against reagent blank at 505 nm (500-540nm) or 505/670 nm on bichromic analyzer. Calculations: Glucose = Absorbance of Test X Concentration of Standard (mg/dl) (mg/dL) Absorbance of Standard ix. Estimation of Bilirubin (BIT BID) Methodology: Diazo Method of Pearlman Lee Principle: Bilirubin reacts with diazotized sulphanilic acid in acidic medium to form pink colored azobilirubin with absorbance directly proportional to Bilirubin concentration. Direct Bilirubin, being water soluble directly reacts in acidic medium. However Indirect or unconjugated Bilirubin is solubilised using a surfactant and then it reacts similar to Direct Bilirubin. Reagent preparation: Procedure: Mix well and incubate for 5 minutes at 370 C for Total Bilirubin and Direct Bilirubin. Read Absorbance at 546/630 nm against Reagent Blank. Calculations with Factors: Total Bilirubin (mg/dl) = Abs. of Test Factor (23). 4.7.2. IN VIVO ANTIOXIDANT PARAMETERS Preparation of homogenate: The homogenate of heart was prepared as follows for the remaining animals. Reagents: 0.25 M sucrose solution: 85.87 g of sucrose was dissolved in 1000 ml of distilled water 10 mM tris buffer solution: 1.2 g of tris was dissolved in 900 ml of distilled water. pH was adjusted to 7.4 with 1M HCl and diluted up to 1000 ml. Procedure: Kidneys were excised and chopped with surgical scalp into fine slices and were chilled in the cold 0.25 M sucrose, quickly blotted with filter paper. The tissue was minced and homogenized in ice cold 10 mM tris HCl buffer (to pH 7.4) at a concentration of 10% (w/v) with 25 stokes of tight teflon pestle of glass homogenizer at a speed of 2500 rpm. The prolonged homogenization under hypotonic condition was designed to disrupt as far as possible the ventricular structure of cells so as to release soluble protein and leave only membrane and non-vascular matter in a sedimentable form. It was then centrifuged at 5000 rpm at 20o C temperature and clear supernatant was separated and used to estimate reduced glutathione (GSH), catalase (CAT) and lipidperoxidation (LPO). a). Catalase (CAT): Catalase was estimated by the method of Hugo E. Aebi method: hydrogen peroxide: hydrogen-peroxidoreductase. Principle: In UV range H2O2 can be followed directly by the decrease in absorbence (O.D 240) per unit time is measure of catalase activity. H2O2 H2 + O2 RDOH H2O + ROH + A Decomposition of H2O2 = Decrease in absorbance at 240 nm Reagents: Phosphate buffer (50 mM, pH 7.0) Dissolve 6.81 g KH2PO4 in distilled water and make up to 1000 ml. Dissolve 8.9 g NaH2PO4. 2H2O in distilled water and make up to 1000 ml. Mix the solution A and B in proportion 1:15 (v/v) Hydrogen peroxide (30 mM/I): Dilute 0.34 ml of 30% Hydrogen peroxide with phosphate buffer up to 100 ml. Procedure: Dilute homogenate 20 times with Phosphate buffer pH 7.0 Calculation: Log (A / B) Ãâ€" 2297.3 Where, A: Initial absorbance B: final absorbance (after 30 second) Units =  µ moles of H2O2 consumed/min/mg b). Reduced glutathione (GSH): Reduced glutathione was determined by the method of Moran et al., 1979. Reagents: TCA (10% w/v) solution: Accurately weighed 10 g of TCA was dissolved in 100 ml of distilled water. Phosphate buffer (0.2 M, pH 8) DTNB reagent (0.6 M): 60 mg of 5,5- dithio bis (2-nitro benzoic acid) was dissolved in 100 ml of 0.2 M sodium phosphate (pH 8). Standard glutathione: Prepared by dissolving 10 mg of reduced glutathione in 100 ml of distilled water. Procedure: To 1 ml of sample, 1 ml of 10% TCA was added. The precipitated fraction was centrifuged and to 0.5 ml supernatant, 2 ml DTNB was added. The final volume was made up to 3 ml with phosphate buffer. The colour developed was read at 412 nm. The amount of glutathione was expressed as  µg of GSH/mg protein, reduced glutathione was used as standard (100  µg/ml). Y – Absorbance of test sample c). Lipid peroxidation: Lipid peroxidation was determined by the method of Slater and Sawsyer et al., 1971 Reagents: Thiobarbituric acid: 0.67% w/v in 1M tris hydrochloride pH -7, 0.67 g of thiobarbituric acid was dissolved in 100 ml of distilled water. Trichloroacetic acid (20% w/v): 20 g of TCA was dissolved in 100 ml of distilled water. Standard malondialdehyde (0-25 n.mol) A stock solution containing 50 mm/ml of 1, 1,3,3-tetra ethoxy propane in tris hydrochloride buffer in pH -7, 10 ml of stock solution was diluted to 100 ml to get a working standard 50 nm malondialdehyde/ml. This was used for preparation of calibration curves. Procedure: 2 ml of sample was mixed with 2 ml of 20% TCA and kept in ice for 15 min. The precipitate was separated by centrifugation and 2 ml of samples of clear supernatant solution were mixed with 2 ml aq. 0.67% TBA solution. This mixture was heated on a boiling water bath for 10 min. It was cooled in ice for 5 min and absorbance was read at 535 nm. The values were expressed as nm of MDA formed/mg of protein values are normalized to protein content of tissues. Y – Absorbance differences of final (after 3 min) and initial reading of test sample.

Research on Hijra Community

Hijra community INTRODUCTION: We know a community is a group of people who work with one another building a sense of trust, care, and support. In Bangladesh Hijra community is a strong community, although they are the most neglected people in our society. Higras are the people who are neither male nor female. In our society they are not treated as human being but hijras claim that they are more capable than men or women. Because they are the combination of both male and female, they are capable of doing the work, which is preferable by men and women.Their demand from society is not so much; they just want to be treated as normal human being and the basic human needs. SUMMARY: 1 (MOVIE: COMMON GENDER) The movie is a reflection of androgen’s life cycle. Androgens were look like men but dressed up like women. So that people teach them and never showed a minimum respect for them. There was one leader of their community who instructed others. One of the characters of this movie was â€Å"Shusmita†. Her parents left her to the hijra community because they think that it was very ashamed things for them that their child was hizra.Shusmita had a male friend named â€Å"Shonjoy† who was also betraying her. After that she felt that there was no meanings of her life for exist. Then she committed suicide. Another character of this film was â€Å"Babu†. The hizra community called her â€Å"Bably†. She always missed her family but if she came home she has been beaten by her brother. Here it is clear that people always keep negative impression about them no matter how hizra communities behave. They collect money from market to survive because societies people never give them work. The most painful part of the movie was people don’t want to entomb hizra with them.This movie focused that androgen people could never have the respect either mortal life or eternal life. SUMMARY: 2 [LIVING ON THE EXTREME MARGIN SOCIAL EXCLUSION OF THE TRANSG ENDER POPULATION (HIJRA) IN BANGLADESH] In childhood if a boy prefers to play with girls, wear female clothing, and household chores then the family members are not take it negatively. Gradually family members notice something goes wrong with their child. Family members felt uncomfortable when the society started to realize the feminine behavior of the boy and comment roughly. There are some families whom are left their child to maintain the social status.But some family kept them. The boy gradually grown up but he cannot change himself because he starts to discover himself that he neither boy nor a girl but a mix of both. He falls into identity crisis as they find feminine emotions trapped inside a manly body. They don’t treat as human because they don’t have any rights, like they cannot take part in the burial of their parents. Lots of situation like this force him to join a hijra community. Over there they find friends like them, so they don’t feel loneliness anymore, and the guru of the community became their parents.She named them newly like â€Å"Salam† become â€Å"Salma†. They get their livelihood and security with and through the community. SUMMARY: 3 They swing between both sexes: hijras as â€Å"asexual others† There are two types of gender male and female, which are classified by our society. But there is another type called â€Å"Hijra† whom is clam that they are third gender. Hijra of Bangladesh defines them as human who are neither male nor female. They can be easily identified by their get-up, language and a very different style of clapping. They developed a distinctive lifestyle gradually.They formed as a community with strong sense of social solidarity. Hijra society of Bangladesh divided into several bands but there is a strong unity and coordination among them. Through every community have separate area, they often visit another community and they welcomed with warmly and greatly treated beca use hijra society is very hospitable. Hijras are mostly illiterate, jobless and homeless. They can barely manage food for survival or see a good doctor for checkup. They have no stable flow of income. In India hijra have a high income rather than Bangladesh.But in our country hijras are fighting hard to survive. They sing and dance for new born baby; they also collect money from market places. If our mainstream society accepts them as normal human being they can seek for new sources of income. QUESTIONNAIRE 1. Where they live? 2. Do you have any community? 3. How they come into the community? 4. Who bring you to the community? 5. Do you have any leader? 6. How a group leader is selected? 7. What is your role? 8. What is your leader’s role? 9. What are their occupation / how they survive? 10. Do you maintain any selected area for collecting money? 1. Did your family still communicate with your? 12. How do you communicate with others? 13. How you get the news of new hizras? 14. What are the requirements to enter the community? 15. If anyone faces any problem, how they solve the problem? 16. Do you have solved the problem by yourself (individual) / other members help you? 17. What is the strength of your community? 18. What they demand from the society? 19. How can you serve the society/ country? 20. Do you have any social or political backup? 21. Which religion do you belong? Do you still maintain it? How? 2. Do you face any problem to maintain it? What are those barriers? Case study: 1 Putuli was another member of Rayer Bazaar hijra community. She told us that she came into this community when she was nine years old. Because of her hormone problem her family left her there. Although her mother loved her lot but unable to stop her father to relinquish her from the family. She told us that still she wanted to stay with her family but when she go for her family they behaved very rude with her. They don’t even touch her and made face like they have se en a alien.Then she told us about their leader on the Rayer Bazaar hijra community who takes care of her as like her child. The leader decided the role of each member and the leader was selecting by concerning their age no matter how active she works. She told us that they dance and sing on society’s birthday party and marriage ceremonies but basically they collect money from market to survive. Then she told that the existence of the society is very tough and painful for her because she always asked herself that who wanted to bag people for money or forced them for giving money for free, her answer is may be no one.It proves that she also have a very strong self-respect. They just don’t understand that it is not any diseases but hormone problem so why people looking at them very oddly with negative point of view. One of the most important things about their community was although they know that they were HIJRA but they didn’t like that word. Then she smilingly t old us that she can handle any kind of problem easily because she has the ability of both male and female. She and her friends can maintain any situation by herself without any help of other by showing their disability approach or brain.This was the main strength of their community that without any political or social backup they live their life with each other. There are different kinds of hijra of their community who belong various religions like Hindu, Muslim, Christian, and Buddha etc. They celebrate their religious occasion like other people of society. Case Study-2 Bala is a Hijra (androgen), who is a member of Rayerbazer hizra community. The size of the community is large, where 65 people live together. They have a leader in this community who is the eldest person of the community, they call her khala, and she plays a vital role in the community.She did not born in the community, she was borrowed from her family when she was 6 years old. Her family doesn’t try to bring her back or stay connected with her. They don’t take any baby because they think if their next baby born as hijra. She told the community has a strong network through they get the news of new hijras. Hijras are easily identifiable because of their nature and attitude. Where all the members along Bala collect money shops and give them all to khala, khala ensure the livelihood for all the members. The brotherhood is so strong between them, if anyone faces any problem everyone comes forward to help.The strength of the community is one for all, all for one. But they never have the happiness of family. They want life partners but that is quite impossible. Her demand from the society is everyone should treat them like a normal human being. Sometime they committed suicide because they cannot handle the way they are treated. She don’t like to collect money from other, she claims that â€Å"I can do any work, like others I also have hands and those are strong. We are not usel ess; we can contribute for the country. We just need opportunity. † They work as the entertainer of the society. But they don’t want to be the clown of the society.They don’t want to live by begging from others. She don’t like the way people look at her. People called her Hijra, which is very painful to her. She wants to known by her name. She came from a Muslim family and still maintains her religion. Like her other members of different religion live together without facing any problem. Case Study-3 Hanna is a member of Keranigonj Hijra community. First time when I saw her she was in a barber shop. She seemed very friendly. I took a short discussion with her. The community where she lives is not a large one. The community is leaded by her guru.She come into this community when she was 12 years old. Her family was ashamed of her. She left her family because of the torture of her father and some of the members of that community offer to join them. Here she found family, friend, food and shelter. After 13 years she is holding a important position in the community. They basically collect money from the local markets and shops. They also participate in the dance of marriage and puja. When they got any information about new born baby they go and dance for money. When I asked about the problem that she faced, she replied there is problem in every aspect of the life of a hijra.One of the problems is people react very oddly when they saw a hira. She said they look like they saw an alien on the street. She handles this problem very interestingly; that she thinks that she is a beauty queen and no one can took their eyes off her. Although they are very confident about themselves but they need the support of their community to survive in the society. People don’t respect them as human being but they have more attachments and feelings, moreover they are very religious. As they are also living human being, they want the basic human needs fr om society.They want to get out of their traditional lifestyle. They want to live in normal human society as a normal human being. They want to work for the society as well as for the country. They are strong and there is no doubt about it, because their community is surviving without any political and social backup. CONCLUSION: Above all it can be say that hijra community who demand themselves as third gender also part of our society. They want nothing but the minimum respect for them and basic rights by which they can survive. If our society provides them chance to do something for betterment they can prove themselves as an asset.We all should help them to develop their community as well as our society by providing the facility which they deserve. It is the right time to change our point of view to them so that they can turn their weakness into strength by serving our country and never hesitate to think that they are also a part of our society. Government should also help them by making laws and policies by serving them with their rights. REFERENCE: †¢http://www. ncbi. nlm. nih. gov/pmc/articles/PMC2928103/ †¢http://www. transgenderasia. org/paper_swinging. htm †¢Movie : common Gender †¢Survey Questionnaire

Norm Paper

PAPER # 1: BREAKING A NORM NAME: Nisha Tommy A norm is a principle of right action binding upon the members of a group and serving to guide, control, or regulates proper and acceptable behavior. Social norms are group-held beliefs about how members should behave in a given context. Sociologists describe norms as informal understandings that govern society’s behaviors, while psychologists have adopted a more general definition, recognizing smaller group units, like a team or an office, may also endorse norms separate or in addition to cultural or societal expectations.The psychological definition emphasizes social norms' behavioral component, stating norms have two dimensions: how much behavior is exhibited and how much the group approves of that behavior. The people living in the society develop social norms. These norms have its importance and pay a vital role in the socialization of an individual and molding of personality. There are some important functions of social norms, which play essential part in the smooth of the society. These are control behavior, harmonize the society, and law and order. I chose to violate a social norm when riding on the elevator.I did this in a hospital environment where doctors, nurses, technicians, and mostly patients were around me. I monitored the reaction of ten different groups throughout the experiment to get the best result. It is included different nationality and different age group. I rode the elevator ten times but instead of standing the â€Å"normal† way which is facing the door an unspoken social norm I stood the wrong way standing by the door looking at the back of the elevator. I felt very uncomfortable for the first few times but, after that I started enjoying people’s nonverbal reactions.Still it is hard to break a norm by knowingly breaking it. I got various reactions. Some people stare at me as if I was not normal. Some rolled their eyes and others startled their forehead. Some of them as ked me if I was ok. Some couples looked each other and smiled. Some shook their head. Some of them didn’t look at me at all they were looking on the floor. One of the employees asked me â€Å"Do you work here? Which department do you work for? † One person asked me â€Å"Do you have elevators in your country†? One person asked me â€Å"how long have you been in US†? After my answer she made a facial expression.The question â€Å"Are you ok? ’’ contradicted my hypothesis and proved it wrong which was even if I stand the wrong way in the elevator and go against the social norm then people still won’t really react. This experiment proved my hypothesis wrong. While I had thought I wouldn’t get any reactions I did in reality get many reactions from staring to being asked if I was ok. Many people stared at me as though there was something wrong with me or even felt uncomfortable around me. My hypothesis was proven wrong because clea rly breaking a social norm is more significant than I had assumed.The experiment proved a lot about social norms and breaking them. It showed the significance of social norms how much social norms play a role in society and people as individuals act. The social norm I broke was implicit yet it proved to be so great in how people saw me and how they reacted. I standing the wrong way changed the way some people felt in the elevator they started to feel uncomfortable because my actions went against the social norm which is standing facing the door and anything that goes against the social norm is not normal.The gesture was small yet it proved to generate greater reactions. This experiment proves that there are such things as social norms that aren’t even formally stated and as a society rules are set as to how once should behave and when broken one is met reactions and seen as different and â€Å"not normal†. Even in fast paced places where people tend to be too busy or t hey are stressed or even too sick to notice, people notice the breaking of social norms and react toward it because that is how people were taught to look at differently those who don’t follow the social norm.I had believed that in United State where the attitude of minding your business is what people shape their lives around this would not be the case but I was wrong. Adhering to social norms is clearly a big part of people’s lives and shapes the way they act. What surprised me about the results was the way people acted toward me how some people were feeling uncomfortable. This surprised me because they were acting like there might be something wrong with me just because I was standing the wrong way. The man who asked me if I am ok shocked me the most because I didn’t expect anyone to actually ask me this.All of this also shows how the environment plays a role in a person’s life. The social norms of a place which differ from place to place shapes the so ciety it sets the social code. Especially in the case of the man who asked me if I was ok. His behavior and the rest of the experimental group showed how me breaking the social norm affects not only me but the people around me and changes the way I am seen to the world. He would have probably not asked me this is I was standing the right way. But because I violated the social norm his behavior and reactions changed.The groups acted differently because of the variable which made all the difference. One can bring in conformity to explain these results. Society as a whole has learned to conform so as not to be the odd one out. The social based rules that were made people as a whole have learned to change their behavior to conform to the universal social code so they aren’t different. And when they see someone who acts differently they see them as â€Å"weird† different. People like behaviorists would say have been â€Å"shaped† a certain way a socially acceptable w hich is why people reacted the way they did to me.I went against everyone the rules of society by not conforming hence I was the odd one out. If it weren’t for the social norms then maybe I would not have gotten the reactions I did because there wouldn’t be anything to conform too. One can infer that some of the reactions showed the following of the social norm of not being rude. While some of them stared they did not do anything as not to be rude which is in its own way following a social norm of politeness. This experiment has taught me basically a lot more about social norms and society. I got to see firsthand how social norms work and how much they play a role in everyone’s daily lives.I learned how people react toward people who don’t follow the social norms and break them. If this experiment was conducted somewhere else I would say definitely the results would be different, because the place makes the social norms. Every environment is different and society makes rule based on that. What might be acceptable in one place might not be so acceptable in another and vice versa. For example in some place standing away at a decent distance is considered disrespectful that is going against the social norm whereas in America that is not the case. Here standing too close is breaking the social norm.The results might have been more drastic or there might have not been any reaction at all. This experiment helped in providing the significance of social norms. The experiment also proved to show the difference between following and breaking social norms. After breaking the social norm I got to see many reactions which proved my hypothesis wrong and showed how conformity has a strong impact on society. By standing backwards in an elevator I broke the social norm of standing facing the back of the elevator and hence I became the odd one out. I enjoyed doing this experiment as a whole.

The Death Of A Salesman By Arthur Miller - 1618 Words

Both the authenticity and the purity of the American Dream have been put into question by various pieces of literature, such as the Death of a Salesman by Arthur Miller, or even the modern classic American Psycho by Bret Easton Ellis. With specific regard to The Great Gatsby by F. Scott Fitzgerald, the death of the American Dream is seen through the eyes of Nick Carraway as he watches America’s morality and virtue disintegrate before him. The American Dream is supposed to represent the ideal that every U.S. citizen should have an equal opportunity to achieve success and prosperity through hard work, determination, and initiative. With that being said, the failure of the American Dream in the novel is epitomized by the lack of equality, moral values, and the ultimate demise of both Gatsby and the dreams for which he represented. Notably, social discrimination is undoubtedly a prominent factor in the downfall of the American Dream. The Valley of Ashes is used as a physical repre sentation of the prominent division between the upper and lower class. The fact that the rich hold such a deep aberration for the poor is further exhibited through the relationship between Tom Buchanan and George Wilson; Tom establishes his unquestionable superiority over Wilson and constantly looks down on him, as demonstrated by the quote, â€Å"He’s so dumb he doesn’t know he’s alive† (26). Myrtle, like her husband, is not immune to Tom’s powerful presence, in fact, she embraces his wealth as if it wasShow MoreRelatedDeath Of A Salesman By Arthur Miller1387 Words   |  6 PagesAmerican play-write Arthur Miller, is undoubtedly Death of a Salesman. Arthur Miller wrote Death of a Salesman in 1949 at the time when America was evolving into an economic powerhouse. Arthur Miller critiques the system of capitalism and he also tells of the reality of the American Dream. Not only does he do these things, but he brings to light the idea of the dysfuncti onal family. Death of a Salesman is one of America’s saddest tragedies. In Arthur Miller’s, Death of a Salesman, three major eventsRead MoreDeath Of A Salesman By Arthur Miller888 Words   |  4 PagesDeath of a Salesman† is a play written by Arthur Miller in the year 1949. The play revolves around a desperate salesman, Willy Loman. Loman is delusioned and most of the things he does make him to appear as a man who is living in his own world away from other people. He is disturbed by the fact that he cannot let go his former self. His wife Linda is sad and lonely; his youngest son Biff is presented as a swinger/player while his eldest son Happy appears anti-business and confused by the behaviorRead MoreDeath Of A Salesman By Arthur Miller1573 Words   |  7 Pagesrepresents a character with a tragic flaw leading to his downfall. 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Arthur Miller puts a modern twist on Aristotle’s definition of ancient Greek tragedy when Willy Loman’s life story directly identifies the fatal flaw of the â€Å"American Dream†. Willy Loman’s tragic flaw can be recappedRead MoreThe Death Of A Salesman By Arthur Miller846 Words   |  4 PagesA Dime a Dozen The Death of a Salesman is a tragedy written by playwright Arthur Miller and told in the third person limited view. The play involves four main characters, Biff, Happy, Linda, and Willy Loman, an ordinary family trying to live the American Dream. Throughout the play however, the family begins to show that through their endeavors to live the American Dream, they are only hurting their selves. The play begins by hinting at Willy’s suicidal attempts as the play begins with Linda askingRead MoreDeath Of A Salesman By Arthur Miller Essay2538 Words   |  11 PagesSurname 1 McCain Student’s Name: Instructor’s Name: Course: Date: Death of a Salesman Death of a salesman is a literature play written by American author Arthur Miller. The play was first published in the year 1949 and premiered on Broadway in the same year. Since then, it has had several performances. It has also received a lot of accordances and won numerous awards for its literature merit including the coveted Pulitzer for drama. The play is regarded by many critics as the perfectRead MoreDeath Of A Salesman By Arthur Miller2081 Words   |  9 Pages#1 â€Å"Death of a Salesman† by Arthur Miller is a tragedy, this play has only two acts and does not include scenes in the acts. Instead of cutting from scene to scene, there is a description of how the lighting focuses on a different place or time-period, which from there, they continue on in a different setting. The play doesn’t go in chronological order. A lot of the play is present in Willy’s flashbacks or memories of events. This provides an explanation of why the characters are acting a certainRead MoreDeath Of Salesman By Arthur Miller1475 Words   |  6 Pagesto death to achieve their so- called American dream. They live alone and there is no love of parents and siblings. They may have not noticed the America dream costs them so much, which will cause a bigger regret later. In the play Death of Salesman, Arthur Miller brings a great story of a man who is at very older age and still works hard to achieve his desire, which is the American dream. Later, he no tices that his youth is gone and there is less energy in his body. Willy Loman is a salesman, who